Isolation and Activity Test of Lipolitic Bacteria on Different pH and Temperature

Lipase is an enzyme that can be applied in many industrial fields so it has a good economic prospects. The demand for lipase was still unfulfilled at the time this article written. character of needs are vary depending on industry which used. Temperature and pH two factors most influence activity. This reports bacteria isolated from coconut, avocado, tempeh, areca nut their lipolytic activity 7-10, 30-40oC. Isolation carried out NA media supplemented with olive oil Rhodamine B as indicator. Colonies showing clear zones were used subsequent work. Activity tests isolation medium 7-10 incubated 30 or 40oC. Characterization isolates includes morphological microscopic observations; proteolytic amylolytic capability. There 41 activity, 26, 7, 4, 4 nut, respectively. Five selected further showed best 30oC K1A1 K1A2 each had index 9% 107%. Meanwhile, 40oC shown by K10 8 59%. Based Grams staining analysis, belong to Gram-positive coccus bacteria. Isolate high but not amylolytic, while did show both activities.